This study continues documentation of the adaptive radiation of species in the genus Tetragnatha in the Hawaiian archipelago. The five new species described here are representatives of the ‘spiny-leg’ clade, most representatives of which have already been described. The new species are T. kukuiki, T. kikokiko, T. anuenue, T. kukuhaa, and T. obscura. The species described are found in various different habitat types from low (e.g., 550 m on Oahu) to middle elevations (1660 m on Hawaii), and from dry to very wet forest types. As with other representatives of the clade, they are nocturnal hunters, do not build webs, and move actively around the vegetation.